ANU - STUDYAT - Molecular Biotechnology

Offered By:

Biochemistry & Molecular Biology

Academic Career:

Undergraduate

Course Subject:

Biology

Offered in:

Second Semester, 2010

Unit Value:

6 units

Course Description:

This course provides an introduction to the principles and practice of recombinant DNA technology. It has a focus on how biological processes can be exploited and manipulated for practical purposes, rather than how they operate in nature. The course also introduces some of the main problem solving skills used routinely in molecular biology. A wide range of methods and applications will be discussed including: DNA cloning, gene libraries, DNA sequencing, polymerase chain reaction (PCR), blotting techniques, expression of recombinant proteins, gene mapping, transgenic animals, and gene therapy.

Learning Outcomes:

On satisfying the requirements of this course, students will have the knowledge and skills to:

1. Describe and apply methods and techniques used to manipulate DNA and generate transgenic (genetically modified) organisms
2. Design strategies for identifying and analysing genes and their function
3. Recognise the different requirements for gene expression in yeast, bacteria, plants and animals
4. Demonstrate practical skills used in molecular biotechnology such as PCR and molecular cloning

Indicative Assessment:

Assessment will be based on:

Tutorial tests (30%; LO 1, 2, 4)
Practical lab exercises (journal and report 20%; LO 1, 3, 4)
Final exam (50%; LO 1, 2, 3)

Workload:

Two lectures per week; five laboratory sessions of up to four hours each; eight one-hour tutorials

Requisite Statement:

BIOL2161 and CHEM1201 (or CHEM1015/17)

Prescribed Texts:

Brown, T.A. (2006) Gene Cloning and Analysis: an introduction (5th ed.) Blackwell

Science Group:

B

Academic Contact:

Dr David Tscharke

Molecular Biotechnology BIOL2162 - Details