ANU - STUDYAT - Tools for Molecular Ecol

Offered By:

Botany and Zoology

Academic Career:

Undergraduate

Course Subject:

Biology

Offered in:

First Semester, 2010

Unit Value:

6 units

Course Description:

This course explores the essential theory underpinning the laboratory tools available for the molecular genetic analysis of individuals, populations and species of microorganisms, especially those causing disease. These methods are also applicable to the genetic analyses of non-disease causing agents e.g. plants and animals.

Topics include:

DNA extraction
RNA extraction
DNA fingerprinting
PCR
PCR based molecular markers including RFLP, AFLP, microsatellites, SNPs
DNA sequencing
Virulence profiling of microorganisms
Multi-locus sequence typing
DNA barcoding
Molecular ecology

The course will also review: the principles of laboratory safety, chemistry for general laboratory analysis and procedures essential for working with bacterial and fungal organisms.

Learning Outcomes:

On satisfying the requirements of this course, students will have the knowledge and skills to:

1. demonstrate knowledge of the principles of laboratory safety
2. understand and describe the range of molecular genetic analysis tools available and evaluate their strengths and limitations for different tasks
3. perform laboratory work under supervision including DNA extraction, PCR and DNA sequencing
4. perform and analyse the outcomes of a laboratory-based mini project, and compile and interpret data generated by this work in written and oral form

Indicative Assessment:

Assessment will be based on:

Theory quizzes (5%; LO 1-2)
Lab book assessment (20%; LO 1,3,4)
Assignment in form of practical report (40%; LO 1-4 )
Oral presentation (10%; LO 4)
Mid-term exam (25 %; LO 2-3)

Workload:

Up to 16 lectures, practical sessions and a large practical project

Requisite Statement:

BIOL2161 and BIOL2151

Recommended Courses:

BIOL3151

Science Group:

C

Academic Contact:

Dr Celeste Linde

Tools for Molecular Ecology BIOL3137 - Details